James C. Matthews, Ph.D.
Dept. of Animal and Food Sciences
213 W. P. Garrigus Building
University of Kentucky
Lexington, KY 40546-0215
Telephone: (859) 257-7513
Telefax: (859) 257-5318
1995 – 1997 Post-doc.
Univ. Florida College of Medicine: Joint appointment in Biochemistry & Molecular Biology/Pediatrics–Gastroenterology
1995 Ph.D. Virginia Tech Major: Animal Science 1992 M.S. Virginia Tech Major: Animal Science 1988 B.S. Rutgers University Major: Animal Science (minor: Nutrition)
PROFESSIONAL APPOINTMENTS (as a University of Kentucky faculty member)
Assistant Professor, Department of Animal Sciences, University of Kentucky (1998 - 2003)
Associate Professor, Department of Animal Sciences, University of Kentucky (2004 - 2012)
UK-Alltech Professor of Applied Nutritional Sciences (2007 - present)
Adjunct Assoc. Professor, Dept. Animal and Poultry Sciences, Univ. of Guelph (2008 - 2011)
Professor, Department of Animal and Food Sciences, University of Kentucky (2012 - present)
Research Program Overview
My program of research in nutritional physiology has focused on the molecular and biochemical
characterization of functional units of nutrient transporters and enzymes, which together achieve
a biochemical capacity. Because of their importance in nutrient assimilation and use, my
research program has emphasized the study of tissue-, age-, diet- (including form of
supplemental selenium), metabolic acidosis, ergot alkaloid-dependent expression and activity of
proteins in ruminants. Based on findings from these commercially-relevant experimental
models, my most recent research approaches include characterization of multiple gene and
protein expression profiles, in addition to understanding how the mechanisms by which the
expression and function of specific proteins is regulated. However, my broad research stratagem
is not to study/characterize complete transcriptomes or proteomes but rather to understand how
constitutive and inducible aspects of biochemical pathways are regulated, in response to
physiological challenges of importance to production agriculture.
Teaching Program Overfview
Graduate - Developed and teach “Physiology of Digestion and Nutrient Absorption” (3 credits, 9 semesters)
Graduate - Developed and teach “Protein Metabolism” (2 credits, 8 semesters).
Graduate - Graduate - Department of Animal and Food Sciences Graduate Seminar (1 credit, 3 semesters)
Undergraduate - Research: mentored 61 credit hours of undergraduate research in my lab.
Graduate Faculty (as sole advisor)
Completed: 7 M.S. and 3 Ph.D. students, and 6 postdoctoral fellowships
Current: 3 Ph.D. students
Reviewer Service (1998 - present)
Journals - Editorial Board Member: J. Anim. Sci. (2001-2004); Ad hoc reviewer: J. Animal Science, J. Dairy Science, J. Biological Chemistry, American J. Physiology, J. Nutrition, J. Nutritional Biochemistry, FEBS Letters, J. Veterinary Medicine, CAST
Grants - Review Panel Member: USDA NIFA Competitive Grants Program; Ad hoc Reviewer: NSF- Integrated Organism Systems, USDA-NRICGP, USDA-BARD, The Wellcome Trust,. Natural Sciences and Engineering Research Council of Canada, National Science Centre of Poland, Vienna Science and Technology Fund.
Critical University of Kentucky Committee Service
Agriculture Biotechnology Program Coordinating Comm. (1999 - 2012); University Radiation Safety Comm. (2002 - 2008); University Biological Safety Comm. (2008 – 2011).
Conceived, organized, and sponsored (as the University of Kentucky-Alltech Professor of Applied Nutritional Sciences) 4 workshops (experimental design, RNA processing, RNA-silencing, bioinformatic analysis, and statistical analyses), nine seminars, and 3 symposiums on nutrigenomics, epigenetics, and microRNA by leading researchers from Harvard, Dartmouth, Univ. of Louisville, Univ. of Illinois, Penn State Univ., Univ. California, The Ohio State Univ., Texas A&M Univ., NIH/NCI, Univ. Texas Health Science Center San Antonio, INRA, and Queen’s Medical Centre. Over 1,200 undergraduate and graduate students, postdoctoral fellows, and faculty from Univ. of Kentucky, Eastern Kentucky Univ., and private industry groups attended these presentations.
RECENT REFEREED MANUSCRIPTS (of 57)
S. F. Liao, J. A. Boling and J. C. Matthews. 2015. Gene expression profiling reveals an increased capacity for proline, serine, and ATP synthesis, and mitochondrial mass, by the liver of steers grazing high vs. low endophyte-infected tall fescue. Journal of Animal Science 93:1-13. doi:10.2527/jas2015-9193.
J. L. Klotz, K. R. Brown, Y. Xue, J. C. Matthews, J. A. Boling, W. R. Burris, L. P. Bush, and J. R. Strickland. 2012. Alterations in Serotonin Receptor-induced Contractility of Bovine Lateral Saphenous Vein in Cattle Grazing Endophyte-infected Tall Fescue. Journal of Animal Science 90:682-693.
E. D. Miles, Y. Xue, J. R. Strickland, J. A. Boling, and J. C. Matthews. 2011. Ergopeptines Bromocriptine and Ergovaline, and Domperidone, Inhibit Bovine Equilibrative Nucleoside Transporter 1-like Activity. Journal of Agriculture and Food Chemistry. 59:9691-9699.
Y. Xue, J. R. Strickland, J. A. Boling, J. C. Matthews. 2011. Bovine Vesicular Glutamate Transporter Activity Is Inhibited by Ergovaline and Other Ergopeptines. Journal of Dairy Science 94:3331-3341.
J. R. Strickland, M. L. Looper, J. C. Matthews, C. F. Rosenkrans, M. D. Flythe, and K. R. Brown. 2011. BOARD-INVITED REVIEW: St. Anthony’s Fire in Livestock: Causes, Mechanisms and Potential Solution. Journal of Animal Science 89:1603-1626.
K. R. Brown, G. A. Anderson, K. Son, G. Rentfrow, L. P. Bush, J. L. Klotz, J. R. Strickland, J. A. Boling, and J. C. Matthews. 2009. Growing Steers Grazing High versus Low Endophyte (Neotyphodium coenophialum)-infected Tall Fescue Have Reduced Serum Enzymes, Increased Hepatic Glucogenic Enzymes, and Reduced Liver and Carcass Mass. Journal of Animal Science 87:748-760.
K. L. Cerny, L. Anderson, W. R. Burris, M. Rhoads, J. C. Matthews, and P. J. Bridges. 2015. Form of supplemental selenium fed to cycling cows affects systemic concentrations of progesterone, but not estradiol. Accepted 10-12-15 by Theriogenology as manuscript no. THERIO-D-15-00521R1.
K. L. Cerny, K. L., S. Garbacik, C. Skees, W. R. Burris, J. C. Matthews, P. J. Bridges. 2015. Gestational form of selenium in free-choice mineral mixes affects transcriptome profiles of neonatal calf testis, including those of steroidogenic and spermatogenic pathways. Biological Trace Element Research doi:10.1007/s12011-01500386-4.
J. C. Matthews, Z. Zhang, J.D. Patterson, P.J. Bridges, A.J. Stromberg, and J.A. Boling. 2014. Hepatic transcriptome profiles differ among maturing beef heifers supplemented with inorganic, organic, or mixed (50% Inorganic:50% Organic) forms of dietary selenium. Biological Trace Element Research 160:321-329.
J. C. Matthews, and P.J. Bridges. 2014. NutriPhysioGenomics applications to identify adaptations of cattle to consumption of ergot alkaloids and inorganic versus organic forms of selenium: altered nutritional, physiological and health states? Animal Production Science 54:1594-1604.
J. D. Patterson, W. R. Burris, J. A. Boling and J. C. Matthews. 2013. Individual Intake of Free-choice Mineral Mix by Grazing Beef Cows May Be Less than Typical Formulation Assumptions and Form of Selenium in Mineral Mix Affects Blood Se Concentrations of Cows and their Suckling Calves. Biological Trace Mineral Research 155:38-48.
K. M. Brennan, W. R. Burris, J. A. Boling and J. C. Matthews. 2011. Selenium Content in Blood Fractions and Liver of Beef Heifers is Greater with a Mix of Inorganic/Organic or Organic Versus Inorganic Supplemental Selenium Forms but the Time Required for Maximal Assimilation is Tissue-specific. Biological Trace Mineral Research, 144:504-516.
S. F. Liao, K. R. Brown, A. J. Stromberg, W. R. Burris, J. A. Boling, and J. C. Matthews. 2010. Dietary Supplementation of Selenium in Inorganic and Organic Forms Differentially and Commonly Alters Blood and Liver Selenium Concentrations and Liver Gene Expression Profiles of Growing Beef Heifers Biological Trace Element Research 140:151-169.
E. D. Miles, B. W. McBride, Y. Yang, S. F. Liao, J. A. Boling, P. J. Bridges, and J. C. Matthews. 2015. Glutamine Synthetase and Alanine Transaminase Expression Are Decreased in Livers of Aged vs. Young Beef Cows and GS can be Up-regulated by 17β-estradiol Implants. Journal of Animal Science 93:4500-4509. doi 10.2527/jas2015-9294.
C. C. Taylor-Edwards, D. G. Burrin, J. C. Matthews, K. R. McLeod, J. J. Holst, and D. L. Harmon. 2010. Expression of mRNA for Proglucagon and Glucagon-like Peptide-2 (GLP-2) Receptor in the Ruminant Gastrointestinal Tract and the Influence of Energy Intake. Domestic Animal Endocrinology, 39:181-193.
S. F. Liao, D. L. Harmon, E. S. Vanzant, K. R. McLeod, J. A. Boling, and J. C. Matthews. 2010. The Small Intestinal Epithelia of Beef Steers Differentially Express Sugar Transporter mRNA in Response to Abomasal vs Ruminal Infusion of Starch Hydrolysate. Journal of Animal Science 88:306-314.
S. F. Liao, E. S. Vanzant, D. L. Harmon, K. R. McLeod, J. A. Boling, and J. C. Matthews. 2009. Ruminal and Abomasal Starch Hydrolysate Infusions Selectively Decrease the Expression of Cationic Amino Acid Transporter mRNA by Small Intestinal Epithelia of Forage-fed Beef Steers. Journal of Dairy Science 92:1124-1135.
S. F. Liao, J. S. Monegue, M. D. Lindemann, G. L. Cromwell, and J. C. Matthews. 2010. Dietary Supplementation of Boron Differentially Affects Expression of Borate Transporter (NaBC1) mRNA by Jejunum and Kidney of Growing Pigs. Biological Trace Element Research 143:901-912.
M. A. Steele, O. AlZahal, L. Dionissopoulus, A. H. Laarman, J. C. Matthews, and B. W. McBride. 2015. The Periparturient Period Is Associated with Structural and Transcriptomic Adaptations of Rumen Papillae in Dairy Cattle. Journal of Dairy Science 98:2583-2595.
L. Dionissopoulus, O. AlZahal, M.A. Steele, J.C. Matthews, and B.W. McBride. 2014. Transcriptomic Changes in the Rumen of Peri-parturient Dairy Cows. American Journal of Animal and Veterinary Sciences 9:36-45.
M. A. Steele, O. AlZahal, S. Greenwood, J. C. Matthews3, and B. W. McBride. 2013. Technical Note: Use of Laser Capture Microdissection for the Localization of Tissue Specific Global Gene Expression in Rumen Papillae. Journal of Dairy Science 96:7748-7752.
M. Steele, G. Vandervoort, O. AlZahal, S. Hook, J. C. Matthews, and B. W. McBride. 2011. Rumen Epithelial Adaptation to High Grain Diets Involves the Coordinated Regulation of Genes Involved in Cholesterol Homeostasis. Physiological Genomics 43:308-316.
Y. Xue, S. F. Liao, K. Son, S. L. Greenwood, B. W. McBride, J. A. Boling, and J. C. Matthews. 2010. Metabolic Acidosis in Sheep Alters Expression of Renal and Skeletal Muscle Amino Acid Enzymes and Transporters. Journal of Animal Science 88:707-717.
N. E. Odongo, S. L. Greenwood, M. M. Or-Rashid, O. AlZahal, A. K. Shoveller, M. I. Lindinger, J. C. Matthews, and B. W. McBride. 2009. Effects of Nutritionally Induced Metabolic Acidosis With or Without Glutamine Infusion on Acid-base Balance, Plasma Amino Acids and Plasma Non-esterified Fatty Acids in Sheep. Journal of Animal Science 87:1077-1084.
GENE EXPRESSION OMNIBUS (GEO microarray datasets)
GSE62570 - Affymetrix WT Btau 4.0 Array (version 1) Gene Chip experiment (16 microarrays) associated with “Pituitary Gene Expression Profiles of Growing Beef Steers Grazing High versus Low Endophyte-Infected Tall Fescue Grass”. James C. Matthews (PI). Released October 23, 2014.
GSE62382 – Affymetrix Bovine Gene 1.0 ST Array (version 1) Gene Chip experiment (13 microarrays) associated with “Neonatal Testis Transcriptome Profiles Differ Among Calves Born to Cows Supplemented with Different Forms of Dietary Selenium Throughout Gestation”. James C. Matthews (Co-PI). Released October 16, 2014.
GSE44680 – Affymetrix WT Btau 4.0 Array (version 1) Gene Chip experiment (36 microarrays) associated with “Hepatic Transcriptome Profiles Differ Among Maturing Beef Heifers Supplemented with Different Forms of Dietary Selenium” James C. Matthews (PI). Released June 1, 2013.
GSE23894 - Affymetrix Bovine Gene Chip experiment (19 microarrays) associated with “Hepatic Gene Expression Profiles of Growing Beef Steers Grazing High versus low Endophyte-infected Tall Fescue Grass”. James C. Matthews (PI). Released November 30, 2010.
GSE19696 - Affymetrix Bovine Gene Chip experiment (18 microarrays) associated with “Dietary Supplementation of Selenium in Inorganic and Organic Forms Differentially and Commonly Alters Blood and Liver Selenium Concentrations and Liver Gene Expression Profiles of Growing Beef Heifers”. James C. Matthews (PI). Released April 19, 2010.
SELECTED INVITED BOOK CHAPTERS (of 10)
B. M. Zanghi* and J. C. Matthews. 2010. Physiological Importance and Mechanisms of Protein Hydrolysate Absorption. In: V. K. Pasupuleti and A. L. Demain (Eds.) Protein Hydrolysates in Biotechnology. Chapter 9, pages 135-177. Springer, Secaucus, New Jersey.
J. C. Matthews, and G. L. Sipe. Patterns and Putative Regulatory Mechanisms of High-Affinity Glutamate Transporter Expression by Ruminants. 2006. Proceedings of the Xth International Symposium on Ruminant Physiology, Copenhagen, Denmark. Pages 263-287.
J. C. Matthews. Expression and Function of Non-Organelle Glutamate Transporters to Support Peripheral Tissue Function. 2005. In: S. Gill and O. Pulido (Eds.) Glutamate Receptors in Peripheral Tissues: Excitatory Transmission Outside the Central Nervous System. Chapter 1, pages 1-30. Kluwer Academc/Plenum Press, New York