University of Kentucky College of Agriculture

Ruminant Nutrition Lab

Major Equipment:

  • UV/VIS spectrophotometer
  • Konelab Clinical analyzer 
  • Atomic absorption spectrophotometer (includes graphite furnace)
  • Technicon Auto Analyzer 
  • HPLC with UV/VIS and fluorescent detection
  • Elementar C-N-analyzer
  • GC-Mass Spectrometer
  • Gas chromatograph with FID
  • Standard and Ankom fiber analysis equipment


Routine Procedures:

Metabolites

  • Standard Blood Chemistry Values for Animals
  • Ruminal Volatile Fatty Acid (VFA)
  • Fecal Volatile Fatty Acid Analysis
  • Blood VFA
  • Derivatization of Short-Chain Fatty Acids in Blood Plasma
  • Derivatization of Lactic Acid for Gas Chomatography
  • Extraction Method for VGA and DIOLS
  • D- And L-Lactate (Enzymatic)
  • Lactic Acid (Barker Summerson)
  • Pyruvate Analysis
  • Acetoacetate Analysis
  • b-Hydroxybutyrate Analysis
  • L-Alanine Analysis
  • Glutamate Analysis
  • Glutamine Analysis
  • Free Fatty Acid Analysis
  • Vitamin E In Plasma
  • Blood Glucose Specific Activity
  • Glucose/Lactate and Glutamate/Glutamine by YSI

Enzymes

  • Amylase
  • Activation of Pancreatic Zymogens Using Enterokinase
  • Trypsin
  • Chymotrypsin
  • Carboxypeptidase A
  • Ruminal Fluid Urease Assay

Nitrogen

  • Kjeldahl Nitrogen And Protein
  • Acid Detergent Insoluble Nitrogen
  • Pepsin Insoluble Nitrogen
  • Protein Assay Using Bca (Pierce, No. 23225)
  • Elementar Nitrogen/Carbon Analyzer
  • Ammonia
  • Preparation of Urin Samples for 15N Urea Enrichment
  • 15N-15N Urea- Sample Preparation and Analysis
  • HPLC Analysis of Available Lysine
  • CCQ-Tag Amino Acid Analysis (PCM WISP)
  • ACCQ-Tag Amino Acid Analysis (Alliance)
  • ACCQ-Tag Sulfur Amino Acid Analysis (Alliance)
  • ACCQ-Tag Sulfur Amino Acid Analysis (PCM WISP)
  • ACCQ-Tag Tryptophan Analysis (Alliance)
  • ACCQ-Tag Tryptophan Analysis (PCM Wisp)
  • Amino Acid Hydrolysis Procedure for Easy Fast Column
  • Quantitation of Plasma Lysine by GC-MS

Fiber

  • Total Dietary Fiber In Foods
  • Neutral Detergent Fiber (Cell Wall Constituents)
  • Alternative Procedure For High Starch Samples
  • Acid Detergent Fiber (ADF)
  • Acid Detergent Lignin (ADL)
  • Filtrations Using Filter Paper For (ADF and NDF )
  • Crucible Cleaning Procedure
  • Oligosaccharides in Feed, Feces and Ileal Degesta by GC
  • Ankom Method For ADF
  • Ankom Method For NDF
  • Ankom Method For Crude Fiber
  • Ankom Method For ADL Using Beakers
  • Calculations for Sequential NDF/ADF Analysis

Markers

  • Titanium Oxide Analysis
  • Acid Insoluble Ash
  • Atomic Absorption Analysis Of Chromic Oxide
  • Analysis Of Chromic Oxide By Wet Ashing
  • Chromic Oxide-Dry Ashing and Colorimetric
  • DTPA Extraction For Rare-Earth Element Analysis
  • Preparation Of Chromium And Cobalt EDTA
  • Indigestible Adf (IADF)
  • Plasma Xylose Assay


Other Analyses

  • Starch Analysis
  • Operation of Parr Model 1756 Oxygen Bomb Calorimeter
  • Total Long Chain Fatty Acid Analysis
  • Analysis Of Total Lipid Content
  • Measurement of Liver Triglyceride Content
  • Inorganic Phosphate Determination
  • Total Calcium Analysis
  • Brush Border Membrane Vesicles
  • Maltase Assay For Transport
  • Alkaline Phosphatase Assay For Transpor
  • Total Sodium Analysis
  • Total Potassium Analysis
  • Manual Analysis of Para-Aminohippuric Acid in Blood
  • Analysis of Phenols and Indoles in Fecal Samples by HPLC
  • Determination of Biogenic Amines in Fecal Samples by HPLC

Microbial Analyses

  • Separation Of Bacteria And Protozoa
  • Bacterial Isolation From Rumen Contents
  • Determination Of D-Alanine
  • Purine Content Of Ruminal Microbes And Digesta
  • Purine Derivatives and Creatinine by HPLC
  • Tilley-Terry Two Stage In Vitro Fermentation
  • Van Soest Modification Of Tilley-Terry Procedure
  • Ankom In Vitro True Digestibility
  • In Vitro Fermentation Using fecal Inoculum
  • In Vitro Gas Production Measurement
  • Determination of Methane Concentration in Gas Samples

Technicon Procedures

  • Procedures for Operation of Technicon Autoanalyzer
  • Para-Amino-Hippuric Acid (PAH) Analysis
  • Urea Nitrogen Analysis
Konelab Analyzer
  • Alpha Amino Nitrogen
  • Amylase
  • Total Protein
  • Ammonia
  • b-Hydroxybutyrate
  • Creatinine
  • Glucose
  • Urea
  • Chloride
  • Potassium
  • Sodium
  • Dipeptidyl Peptidase IV in Bovine Plasma
  • Para Amino Benzoic Acid (PABA)
  • Para Amino Hippuric Acid (pAH)
  • Non-Esterified Fatty Acids (NEFA)

Location of Lab:
Room 816 
W. P. Garrigus Building

Contact Person(s):
David L. Harmon (859)257-7516