James C. Matthews, Ph.D.

James Matthews

Professor
Instruction
Research

jmatthew@uky.edu
Phone (859) 257-7513
Fax (859) 257-5318

Department of Animal and Food Sciences
207 W. P. Garrigus Building
Lexington KY 40546-0215

Specialties
Nutrition
Physiology

Curriculum Vitae
PDF icon matthews_cv_pdf_5-16-2018.pdf

Professional Profile

Professor

Education

1995 – 1997 Post-doc. Univ. Florida College of Medicine: Joint appointment in Biochemistry & Molecular Biology/Pediatrics–Gastroenterology

1995 Ph.D. Virginia Tech Major: Animal Science

1992 M.S. Virginia Tech Major: Animal Science

1988 B.S. Rutgers University Major: Animal Science (minor: Nutrition)

Research

Professor, Department of Animal and Food Sciences, University of Kentucky (2012 - present)

Associate Professor, Department of Animal Sciences, University of Kentucky (2004 - 2012)

UK-Alltech Professor of Applied Nutritional Sciences (2007 - 2014)

Adjunct Assoc. Professor, Dept. Animal and Poultry Sciences, Univ. of Guelph (2008 - 2011)

Assistant Professor, Department of Animal Sciences, University of Kentucky (1998 - 2003)

Research Program Overview

My program of research in nutritional physiology has focused on the molecular study and characterization of nutrient transporters, and enzymes that either produce or metabolize transporter substrates. Because of their importance in nutrient assimilation and use, my program has emphasized the study of tissue-, age-, diet-, metabolic acidosis, and ergot alkaloid-dependent expression and activity of these proteins in ruminants. Based on findings from these commercially-relevant experimental models, my most recent research interests and efforts have become focused on identifying if and how the energy and/or nitrogen status of cells regulates/coordinates the expression and function of enzyme/transporter "functional units". Accordingly, all of my current research projects include targeted and multiple gene and protein expression profiling. However, my broad research stratagem is not to study/characterize complete transcriptomes or proteomes but rather to understand how constitutive and inducible aspects of specific targeted biochemical pathways are regulated, in response to physiological challenges of importance to production agriculture. My current research foci are the use of different forms of selenium in mineral mixes to ameliorate the negative effects of fescue toxicosis on growth and fertility, and to understand the regulation of amino acid transporters and metabolizing enzymes to support the development and fattening of cattle.

Teaching Program Overview

Graduate - Developed and teach “Physiology of Digestion and Nutrient Absorption” (3 credits, 12 semesters)

Graduate - Developed and teach “Protein Metabolism” (2 credits, 10 semesters).

Graduate - Graduate - Department of Animal and Food Sciences Graduate Seminar (1 credit, 12 semesters)

Undergraduate - Research: mentored 67 credit hours of undergraduate research in my lab.

Graduate Faculty (as sole advisor)

Completed: 7 M.S. and 4 Ph.D. students, and 6 postdoctoral fellowships

Current: 2 Ph.D. students

Seeking (May 2018): 1 -2 M.S. or Ph.D. students

Synergetic Activities

Reviewer Service (1998 - present)

Journals - Editorial Board Member: J. Anim. Sci. (2001-2004); Ad hoc reviewer: Science Reports, Amino Acids, BMC Genomics, PLoS ONE, Physiological Genomics, J. Anim. Sci., J. Anim. Sci. & Biotech., J. Biol. Chem., J. Dairy Sci., J. Physiol., J. Nutrition, J. Nutritional Biochemistry

Grants - Review Panel Member: USDA NIFA Competitive Grants Program; Ad hoc Reviewer: NSF- Integrated Organism Systems, USDA-NRICGP, USDA-BARD, The Wellcome Trust,. Natural Sciences and Engineering Research Council of Canada, National Science Centre of Poland, Vienna Science and Technology Fund.

Critical University of Kentucky Committee Service

Agriculture Biotechnology Program Coordinating Comm. (1999 - 2012); University Radiation Safety Comm. (2002 - 2008); University Biological Safety Comm. (2008 – 2011), University Senate Advisory Comm. – Faculty Code (2017 - 2018).

Knowledge Transfer

Conceived, organized, and sponsored (as the University of Kentucky-Alltech Professor of Applied Nutritional Sciences) 4 workshops (experimental design, RNA processing, RNA-silencing, bioinformatic analysis, and statistical analyses), nine seminars, and 3 symposiums on nutrigenomics, epigenetics, and microRNA by leading researchers from Harvard, Dartmouth, Univ. of Louisville, Univ. of Illinois, Penn State Univ., Univ. California, The Ohio State Univ., Texas A&M Univ., NIH/NCI, Univ. Texas Health Science Center San Antonio, INRA, and Queen’s Medical Centre. Over 1,200 undergraduate and graduate students, postdoctoral fellows, and faculty from Univ. of Kentucky, Eastern Kentucky Univ., and private industry groups attended these presentations.

Recent Refereed Manuscripts (of 64)

Fescue Toxicosis

Y. Jia, Q. Li, W. R. Burris, G. E. Aiken, P. J. Bridges, and J. C. Matthews. 2018. Forms of Selenium in Vitamin-mineral Mixes Differentially Affect Serum Prolactin Concentrations and Hepatic Glutamine Synthetase Activity of Steers Grazing Endophyte-infected Tall Fescue. Journal of Animal Science 96:715-727. doi.org/10.1093/jas/skx068.

Q. Li, R. Hegge, P. J. Bridges, and J. C. Matthews. 2017. Pituitary Genomic Expression Profiles of Steers Are Altered by Grazing of High vs. Low Endophyte-infected Forages. PLOS ONE 12:e0184612. https://doi.org/10.1371/journal.pone.0184612.

J. J. Jackson, M. D. Lindemann, J. A. Boling, and J. C. Matthews. 2015. Summer-long Grazing of High versus Low Endophyte (Neotyphodium coenophialum)-infected Tall Fescue by Growing Beef Steers Results in Distinct Temporal Blood Analyte Response Patterns, with Poor Correlation to Serum Prolactin Levels. Frontiers in Veterinary Science doi:10.3389/fvets.2015.00077.

S. F. Liao, J. A. Boling and J. C. Matthews. 2015. Gene expression profiling reveals an increased capacity for proline, serine, and ATP synthesis, and mitochondrial mass, by the liver of steers grazing high vs. low endophyte-infected tall fescue. Journal of Animal Science 93:1-13. doi:10.2527/jas2015-9193.

J. L. Klotz, K. R. Brown, Y. Xue, J. C. Matthews, J. A. Boling, W. R. Burris, L. P. Bush, and J. R. Strickland. 2012. Alterations in Serotonin Receptor-induced Contractility of Bovine Lateral Saphenous Vein in Cattle Grazing Endophyte-infected Tall Fescue. Journal of Animal Science 90:682-693.

E. D. Miles, Y. Xue, J. R. Strickland, J. A. Boling, and J. C. Matthews. 2011. Ergopeptines Bromocriptine and Ergovaline, and Domperidone, Inhibit Bovine Equilibrative Nucleoside Transporter 1-like Activity. Journal of Agriculture and Food Chemistry. 59:9691-9699.

Y. Xue, J. R. Strickland, J. A. Boling, J. C. Matthews. 2011. Bovine Vesicular Glutamate Transporter Activity Is Inhibited by Ergovaline and Other Ergopeptines. Journal of Dairy Science 94:3331-3341.

J. R. Strickland, M. L. Looper, J. C. Matthews, C. F. Rosenkrans, M. D. Flythe, and K. R. Brown. 2011. BOARD-INVITED REVIEW: St. Anthony’s Fire in Livestock: Causes, Mechanisms and Potential Solution. Journal of Animal Science 89:1603-1626.

K. R. Brown, G. A. Anderson, K. Son, G. Rentfrow, L. P. Bush, J. L. Klotz, J. R. Strickland, J. A. Boling, and J. C. Matthews. 2009. Growing Steers Grazing High versus Low Endophyte (Neotyphodium coenophialum)-infected Tall Fescue Have Reduced Serum Enzymes, Increased Hepatic Glucogenic Enzymes, and Reduced Liver and Carcass Mass. Journal of Animal Science 87:748-760.

Selenium Supplementation

Y. Jia, Q. Li, W. R. Burris, G. E. Aiken, P. J. Bridges, and J. C. Matthews. 2018. Forms of Selenium in Vitamin-mineral Mixes Differentially Affect Serum Prolactin Concentrations and Hepatic Glutamine Synthetase Activity of Steers Grazing Endophyte-infected Tall Fescue. Journal of Animal Science 96:715-727. doi.org/10.1093/jas/skx068.

K. L. Cerny, L. Anderson, W. R. Burris, M. Rhoads, J. C. Matthews, and P. J. Bridges. 2015. Form of supplemental selenium fed to cycling cows affects systemic concentrations of progesterone, but not estradiol. Accepted 10-12-15 by Theriogenology as manuscript no. THERIO-D-15-00521R1.

K. L. Cerny, K. L., S. Garbacik, C. Skees, W. R. Burris, J. C. Matthews, P. J. Bridges. 2015. Gestational form of selenium in free-choice mineral mixes affects transcriptome profiles of neonatal calf testis, including those of steroidogenic and spermatogenic pathways. Biological Trace Element Research doi:10.1007/s12011-01500386-4.

J. C. Matthews, Z. Zhang, J.D. Patterson, P.J. Bridges, A.J. Stromberg, and J.A. Boling. 2014. Hepatic transcriptome profiles differ among maturing beef heifers supplemented with inorganic, organic, or mixed (50% Inorganic:50% Organic) forms of dietary selenium. Biological Trace Element Research 160:321-329.

J. C. Matthews, and P.J. Bridges. 2014. NutriPhysioGenomics applications to identify adaptations of cattle to consumption of ergot alkaloids and inorganic versus organic forms of selenium: altered nutritional, physiological and health states?  Animal Production Science 54:1594-1604.

J. D. Patterson, W. R. Burris, J. A. Boling and J. C. Matthews. 2013. Individual Intake of Free-choice Mineral Mix by Grazing Beef Cows May Be Less than Typical Formulation Assumptions and Form of Selenium in Mineral Mix Affects Blood Se Concentrations of Cows and their Suckling Calves. Biological Trace Mineral Research 155:38-48.

K. M. Brennan, W. R. Burris, J. A. Boling and J. C. Matthews. 2011. Selenium Content in Blood Fractions and Liver of Beef Heifers is Greater with a Mix of Inorganic/Organic or Organic Versus Inorganic Supplemental Selenium Forms but the Time Required for Maximal Assimilation is Tissue-specific. Biological Trace Mineral Research, 144:504-516.

S. F. Liao, K. R. Brown, A. J. Stromberg, W. R. Burris, J. A. Boling, and J. C. Matthews. 2010. Dietary Supplementation of Selenium in Inorganic and Organic Forms Differentially and Commonly Alters Blood and Liver Selenium Concentrations and Liver Gene Expression Profiles of Growing Beef Heifers Biological Trace Element Research 140:151-169.

Development Physiology

J. Huang, Y. Jia, Q. Li, W. R. Burris, P. J. Bridges, and J. C. Matthews. 2018. Hepatic Glutamate Transport and Glutamine Synthesis Capacities Are Decreased in Finished Vs. Growing Beef Steers, Concomitant with Increased GTRAP3-18 Content. Amino Acids 50:153-125. doi :10.1007/s00726-018-2540-8.

K. L. Cerny, Q. Li, J. C. Matthews, P. J. Bridges. 2017. Effect of Lipopolysachharide (LPS) on the Expression of Inflammatory mRNAs and MicroRNAs in the Mouse Oviduct. Reproduction, Fertility and Development. doi: 10.1071/RD117241.

E. D. Miles, B. W. McBride, J. A. Boling, P. J. Bridges, and J.C. Matthews. 2017. Effect of 17β-estradiol administration on hepatic glutamine synthetase, β-catenin, and GPR30 in young and aged beef cows. Canadian Journal of Animal Sciences doi: 10.1139/CJAS-2016-0002.

J. C. Matthews, J. Huang, and G. Rentfrow. 2016. High-affinity Glutamate Transporter and Glutamine Synthetase Content in Longissimus Dorsi and Adipose Tissues of Growing Angus Steers Differs among Suckling, Weanling, Backgrounding, and Finishing Production Stages. Journal of Animal Science, in press manuscript 9901.

E. D. Miles, B. W. McBride, Y. Yang, S. F. Liao, J. A. Boling, P. J. Bridges, and J. C. Matthews. 2015. Glutamine Synthetase and Alanine Transaminase Expression Are Decreased in Livers of Aged vs. Young Beef Cows and GS can be Up-regulated by 17β-estradiol Implants. Journal of Animal Science 93:4500-4509. doi 10.2527/jas2015-9294.

C. C. Taylor-Edwards, D. G. Burrin, J. C. Matthews, K. R. McLeod, J. J. Holst, and D. L. Harmon. 2010. Expression of mRNA for Proglucagon and Glucagon-like Peptide-2 (GLP-2) Receptor in the Ruminant Gastrointestinal Tract and the Influence of Energy Intake. Domestic Animal Endocrinology, 39:181-193.

S. F. Liao, D. L. Harmon, E. S. Vanzant, K. R. McLeod, J. A. Boling, and J. C. Matthews. 2010. The Small Intestinal Epithelia of Beef Steers Differentially Express Sugar Transporter mRNA in Response to Abomasal vs Ruminal Infusion of Starch Hydrolysate. Journal of Animal Science 88:306-314.

S. F. Liao, E. S. Vanzant, D. L. Harmon, K. R. McLeod, J. A. Boling, and J. C. Matthews. 2009. Ruminal and Abomasal Starch Hydrolysate Infusions Selectively Decrease the Expression of Cationic Amino Acid Transporter mRNA by Small Intestinal Epithelia of Forage-fed Beef Steers. Journal of Dairy Science 92:1124-1135.

S. F. Liao, J. S. Monegue, M. D. Lindemann, G. L. Cromwell, and J. C. Matthews. 2010. Dietary Supplementation of Boron Differentially Affects Expression of Borate Transporter (NaBC1) mRNA by Jejunum and Kidney of Growing Pigs. Biological Trace Element Research 143:901-912.

Metabolic Acidosis

M. A. Steele, O. AlZahal, L. Dionissopoulus, A. H. Laarman, J. C. Matthews, and B. W. McBride.  2015.  The Periparturient Period Is Associated with Structural and Transcriptomic Adaptations of Rumen Papillae in Dairy Cattle. Journal of Dairy Science 98:2583-2595.

L. Dionissopoulus, O. AlZahal, M.A. Steele, J.C. Matthews, and B.W. McBride. 2014. Transcriptomic Changes in the Rumen of Peri-parturient Dairy Cows. American Journal of Animal and Veterinary Sciences 9:36-45.

M. A. Steele, O. AlZahal, S. Greenwood, J. C. Matthews3, and B. W. McBride. 2013. Technical Note: Use of Laser Capture Microdissection for the Localization of Tissue Specific Global Gene Expression in Rumen Papillae. Journal of Dairy Science 96:7748-7752.

M. Steele, G. Vandervoort, O. AlZahal, S. Hook, J. C. Matthews, and B. W. McBride. 2011. Rumen Epithelial Adaptation to High Grain Diets Involves the Coordinated Regulation of Genes Involved in Cholesterol Homeostasis. Physiological Genomics 43:308-316.

Y. Xue, S. F. Liao, K. Son, S. L. Greenwood, B. W. McBride, J. A. Boling, and J. C. Matthews. 2010. Metabolic Acidosis in Sheep Alters Expression of Renal and Skeletal Muscle Amino Acid Enzymes and Transporters. Journal of Animal Science 88:707-717.

N. E. Odongo, S. L. Greenwood, M. M. Or-Rashid, O. AlZahal, A. K. Shoveller, M. I. Lindinger, J. C. Matthews, and B. W. McBride. 2009. Effects of Nutritionally Induced Metabolic Acidosis With or Without Glutamine Infusion on Acid-base Balance, Plasma Amino Acids and Plasma Non-esterified Fatty Acids in Sheep. Journal of Animal Science 87:1077-1084.

Gene Expression Omnibus (GEO microarray datasets)

GSE107881 - Affymetrix WT Btau 4.0 Array (version 1) Gene Chip experiment (16 microarrays) associated with "Hepatic Gene Expression Profiles of Growing versus Finishing Beef Steers". James C. Matthews (PI). Released December 11, 2017.

GSE62570 - Affymetrix WT Btau 4.0 Array (version 1) Gene Chip experiment (16 microarrays) associated with “Pituitary Gene Expression Profiles of Growing Beef Steers Grazing High versus Low Endophyte-Infected Tall Fescue Grass”. James C. Matthews (PI). Released October 23, 2014.

GSE62382 – Affymetrix Bovine Gene 1.0 ST Array (version 1) Gene Chip experiment (13 microarrays) associated with “Neonatal Testis Transcriptome Profiles Differ Among Calves Born to Cows Supplemented with Different Forms of Dietary Selenium Throughout Gestation”. James C. Matthews (Co-PI). Released October 16, 2014.

GSE44680 – Affymetrix WT Btau 4.0 Array (version 1) Gene Chip experiment (36 microarrays) associated with “Hepatic Transcriptome Profiles Differ Among Maturing Beef Heifers Supplemented with Different Forms of Dietary Selenium” James C. Matthews (PI). Released June 1, 2013.

GSE23894 - Affymetrix Bovine Gene Chip experiment (19 microarrays) associated with “Hepatic Gene Expression Profiles of Growing Beef Steers Grazing High versus low Endophyte-infected Tall Fescue Grass”. James C. Matthews (PI).  Released November 30, 2010. 

GSE19696 - Affymetrix Bovine Gene Chip experiment (18 microarrays) associated with “Dietary Supplementation of Selenium in Inorganic and Organic Forms Differentially and Commonly Alters Blood and Liver Selenium Concentrations and Liver Gene Expression Profiles of Growing Beef Heifers”. James C. Matthews (PI). Released April 19, 2010.

GSE17849 - Affymetrix Bovine Gene Chip experiment (12 microarrays) associated with “Effect of Dietary Grain on Rumen Papillae Gene Expression in Holstein Dairy Cows”. James C. Matthews (Co-PI). Released August 28, 2009. 

Selected Invited Book Chapters (of 10)

B. M. Zanghi and J. C. Matthews. 2010. Physiological Importance and Mechanisms of Protein Hydrolysate Absorption. In: V. K. Pasupuleti and A. L. Demain (Eds.) Protein Hydrolysates in Biotechnology. Chapter 9, pages 135-177. Springer, Secaucus, New Jersey.

J. C. Matthews, and G. L. Sipe. Patterns and Putative Regulatory Mechanisms of High-Affinity Glutamate Transporter Expression by Ruminants. 2006. Proceedings of the Xth International Symposium on Ruminant Physiology, Copenhagen, Denmark. Pages 263-287.

J. C. Matthews. Expression and Function of Non-Organelle Glutamate Transporters to Support Peripheral Tissue Function. 2005. In: S. Gill and O. Pulido (Eds.) Glutamate Receptors in Peripheral Tissues: Excitatory Transmission Outside the Central Nervous System. Chapter 1, pages 1-30. Kluwer Academc/Plenum Press, New York