INDEX

Dairy Chemistry 
Equine Nutrition
Food Chemistry 
Food Microbiology
Food Protein Lab
Mammalian Reproduction 
Nutrient Transport 
Poultry Nutrition 
Reproductive Physiology 
Ruminant Nutrition
Swine Nutrition 


Reproductive Physiology Lab

Major Equipment:

  • Liquid Scintillation Counter
  • High capacity, low speed refrigerated centrifuge
  • Automated' pipetting stations
  • Dubnoff metabolic shaking incubators
  • Assorted equipment for agarose and polyacrylamide gel
  • Electrophoresis and blotting procedures

Procedures:

  • Radioimmunoassay
  • Short term tissue culture
  • Northern and Western blotting

Location of Lab: 419 WPG
Contact Person(s):
Susan Hayes 7-4168

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Mammalian Reproduction Lab

  • Major Equipment:
  • Research microscope
  • Water-jacketed incubator
  • Shaking incubator
  • Thermal cycler
  • Imaging system
  • Spectrophotometer
  • Refrigerated centrifuge

Routine Procedures:

  • Genomic DNA isolation
  • Protein isolation
  • Total RNA isolation, purification and quantification
  • Reverse transcription and PCR

Location of Lab: 218B WPG
Contact Person(s):
Phillip Bridges; 7-4877

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Nutrient Transport Lab

Major equipment:

  • HTS 7000 spectrophotometer (6- to 392-well microtiter plate reader; UV, absorbance, fluoresence reading capabilities).
  • CO2 incubators
  • Cell culture hood
  • Hybridization oven
  • UV crosslinker
  • Gel electrophoresis units (horizontal/agarose, and vertical/PAGE)
  • Superspeed centrifuge (1.5, 10, 50, 250, 500 mL tube or bottle rotors)

Routine procedures:

  • RNA (A260, torulla yeast RNA std) quantification
  • DNA (A260, salmon sperm DNA) quantification
  • Protein (A590, bovine serum albumin; Lowry) quantification
  • Reverse transcriptase
  • PCR
  • TA cloning
  • Enzymatic restriction of cDNA
  • Northern and immunoblot (Western)
  • Primary and immortalized cell culture
  • Whole-cell transport

Location of Lab: 821A WPG
Contact Person(s):
Jamie Matthews 7-7513

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Poultry Nutrition Lab

Major Equipment:

  • Bomb calorimeter
  • Filter fluorometer

Routine procedures:

  • Selenium assay

Location of Lab: 621 WPG
Contact Person(s):
Austin Cantor 7-7531

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Equine Nutrition Laboratory

Major Equipment:

  • YSI Glucose/Lactate Analyzer
  • Refrigerated Centrifuge
  • -80 Freezer
  • Brinkman Homogenizer
  • UV-Visible Spectrophotometer
  • Scanning Thermocouple
  • Portable Large Animal Scale (2000 lb capacity)
  • Large Animal Treadmill (at Farm)
  • Portable Radiograph
  • Equine Heart Rate Monitor
  • Water bath, balances, etc

Routine Procedures:

  • Blood/plasma/serum chemistries (glucose, lactate, free fatty acids, triglycerides, glycerol)
  • Total protein
  • Plasma volume
  • Serum CK and AST
  • Radiographic bone density

Location of Lab: 616 WPG
Contact Person(s):
L. Lawrence 7-7509; Room 905
S. Hayes 7-4168; Room 419

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Food Microbiology Lab

  • Major Equipment:
  • General microbiological equipment
  • Autoclaves
  • Incubators
  • Water Baths
  • PCR Thermal Cycler
  • Laminar Flow Hood

Routine Procedures:

  • Microbial Enumeration
  • Microbial Identification
  • Microbial Challenge studies
  • Antibiotic Resistance studies
  • Fermentation studies

Location of Lab: 221 WPG
Contact Person(s):
Melissa Newman 7-5881

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Food Protein Lab

Major Equipment

  • Bohlin Rheometer
  • Differential Scanning Calorimeter
  • Fluoromax Flourometer
  • Brinkman Polytron
  • Turner Fluorometer
  • LKB Laser Densitometer
  • Perkin Elmer Spectrophotometer
  • Biorad SDS-Page
  • Circulating Waterbath
  • Instron 4301

Routine Procedures

  • Protein Isolation/Purification
  • SDS-Page
  • DSC
  • Sensory Panels
  • Gel Strength
  • Dynamic Rheological Properties
  • Water Holding Capacity
  • Spectrophotometric Analysis
  • Protein Biochemistry Analysis

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Dairy Chemistry Lab

Major Equipment:

  • Babcock test equipment
  • Some membrane equipment
  • Spray drying equipment
  • Instron

Routine Procedures:

  • Babcock fat test

Location of Lab: 216C & D WPG
Contact person(s):
C. Hicks 7-7538

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Swine Nutrition Lab

Major Equipment:

  • Bomb calorimeter (Parr)
  • Nanopure water purifier
  • Microwave digestion (2 units)
  • ELISA plate reader
  • Tecator Soxtec system reflux fat extraction unit
  • Instron machine

Location of Lab: 618/621 WPG
Contact person(s):
Dr. Noel Inocencio 7-7561

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Food Chemistry Lab

Major Equipment:

  • Gas Chromatograph with FID
  • GC/Mass Spectrometer
  • Microbalance
  • Large volume centrifuge (8,000 x g)
  • HPLC with diode array detector
  • UV/VIS spectrophotometer

Routine Procedures:

  • Flavor chemistry procedures

Contact Person(s):
Luke Boatright 7-5988

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Ruminant Nutrition Lab

Major Equipment:

  • UV/VIS spectrophotometer
  • COBAS centrifugal analyzer
  • Atomic absorption spectrophotometer (includes graphite furnace)
  • HPLC with UV/VIS and fluorescent detection
  • LECO N-analyzer
  • Gas chromatograph with FID
  • Standard and Ankom fiber analysis equipment

Routine Procedures:
Metabolites

  • Ruminal Volatile Fatty Acid (VFA)
  • Blood VFA
  • D- And L-Lactate (Enzymatic)
  • Lactic Acid (Barker Summerson)
  • Pyruvate Analysis
  • Acetoacetate Analysis
  • b-Hydroxybutyrate Analysis
  • L-Alanine Analysis
  • Glutamate Analysis
  • Glutamine Analysis
  • Free Fatty Acid Analysis
  • Vitamin E In Plasma
  • Blood Glucose Specific Activity

Enzymes

  • Amylase
  • Trypsin
  • Chymotrypsin
  • Carboxypeptidase A
  • Ruminal Fluid Urease Assay

Nitrogen

  • Kjeldahl Nitrogen And Protein
  • Acid Detergent Insoluble Nitrogen
  • Pepsin Insoluble Nitrogen
  • Protein Assay Using Bca (Pierce, No. 23225)
  • Ammonia

Fiber

  • Total Dietary Fiber In Foods
  • Neutral Detergent Fiber (Cell Wall Constituents)
  • Alternative Procedure For High Starch Samples
  • Acid Detergent Fiber (ADF)
  • Acid Detergent Lignin (ADL)
  • Filtrations Using Filter Paper For (ADF and NDF )
  • Crucible Cleaning Procedure
  • Ankom Method For ADF
  • Ankom Method For NDF
  • Ankom Method For Crude Fiber
  • Ankom Method For ADL Using Beakers

Markers

  • Acid Insoluble Ash
  • Atomic Absorption Analysis Of Chromic Oxide
  • Analysis Of Chromic Oxide By Wet Ashing
  • Chromic Oxide-Dry Ashing And Colorimetric
  • DTPA Extraction For Rare-Earth Element Analysis
  • Preparation Of Chromium And Cobalt EDTA
  • Indigestible Adf (IADF)

Other Analyses

  • Starch Analysis
  • Total Long Chain Fatty Acid Analysis
  • Analysis Of Total Lipid Content
  • Inorganic Phosphate Determination
  • Total Calcium Analysis
  • Brush Border Membrane Vesicles
  • Maltase Assay For Transport
  • Alkaline Phosphatase Assay For Transport

Microbial Analyses

  • Separation Of Bacteria And Protozoa
  • Bacterial Isolation From Rumen Contents
  • Determination Of D-Alanine
  • Purine Content Of Ruminal Microbes And Digesta
  • Tilley-Terry Two Stage In Vitro Fermentation
  • Van Soest Modification Of Tilley-Terry Procedure
  • Ankom In Vitro True Digestibility

Cobas Procedures

  • Measuring Absorbance At 280 Nm
  • Alpha Amino Nitrogen
  • Alpha Amino Nitrogen (Hydrolysates)
  • Plasma Amylase
  • Ammonia Nitrogen Using Gdh For Plasma
  • Ammonia Nitrogen Using Salicylate
  • Bca Soluble Protein
  • b-Hydroxybutyrate In Blood
  • Cholesterol In Plasma
  • Creatinine For Plasma And Urine
  • Chymotrypsin In Pancreatic Juice
  • Plasma Glucose Using Hexokinase
  • Inorganic Phosphorus
  • Free Fatty Acids In Plasma
  • Pah Analysis
  • Trypsin In Bovine Pancreatic Juice

Contact Person(s):
David L. Harmon 7-7516
Dani True 7-2977

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